DNA Extraction from Frozen Tissue Sections

Tissue collection, storage, microdissection, sectioning: See separate protocol. Tissue handling: Note that all fresh tissue should be handled as BioSafety Level 2 materials (wear gloves, lab coat, etc.). DNA extraction: The following protocol is based on a standard phenol DNA extraction protocol. Other protocols, and versions of this protocol, are also acceptable. 1. Take pre-cut samples out of the –80°C freezer and thaw. 2. Add 10 ml of PBS (Ca-Mg Free) in hood in BSL2 room to dissolve OCT. Invert tube to make sure all of tissue
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