Embryo Lysates & Immunoprecipitation

Embryo lysates Take 25 embryos and place into 1.7ml centrifuge tube. Rinse once in lysis buffer (add ~ 1ml) and remove by aspiration Add 500 µL lysis buffer per embryo and homogenize with either P1000 tip. Add 1000 µL freon -- vortex spin in cold room, top speed - 10-15 minutes take the supernatant - either freeze at -80°C or use immediately for blot, immunoprecipitation, DNA-fishing or whatever else suits your fancy. For blots, heat at 80°C for 5 minutes (remember to add 2ME) Load onto gel. Normally 1 embryo equivalent will be more than enough per lane for whole lysates, altho
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