Protein Staining Procedures

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.Coomassie Blue Staining TIP: All washes and incubations are done with constant, gentle shaking.Immerse gel in 50% ethanol/10% acetic acid for at least 1 hr. Soak in 5% ethanol/5% acetic acid overnight or for a minimum of 2 hours. Wash in diH2O for 1 hr. Add Gel-Code Blue Stain reagent (Pierce, #24592) for at least 3 hrs. Wash in diH2O twice, 15 min each. Rinse in
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