Immunoblot Protocol

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.1. MaterialsProtease inhibitor cocktail (Boehringer Mannheim) 2X SDS sample buffer (Novex) Pre-casted gels (e.g. Novex) Molecular weight protein standard (e.g. MultiMark Multi-Colored Standard, Novex) 25X Tris-Glycine transfer buffer (e.g. Novex) 100% methanol Blocking solution containing casein (e.g., Pierce) Wash Solution I (for 1L: 1g Ovalbumin (Sigma), 1g Dry
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