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siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western blotting. This allows us to test several candidate siRNAs quite cheaply. The yields are good enough for reasonable amounts of experimental work, but for larger use we get successful siRNAs synthesised chemically by Dharmacon.DesignThe Tuschl lab siRNA users guide gives information about how to choose the basic target sequence. For enzymatic synthesis, the target sequences for the preparation of T7 polymerase transcribed siRNA are of th
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