Gel Mobility Shift Assay Conditions

Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and BufferSteve Hahnlast modified Mon, Nov 9, 1998Protein Dilution Buffer5ml20 mM Tris pH7.9100 microliters 1M Tris 7.9150 mM KCl0.75 ml 1 M KCl1 mM DTT50 microliters 0.1 M DTT10% glycerol1 ml 50% glycerol50 micrograms/ml BSA2.5 microliters 100 mg/ml BSA 3.1 ml H2OOptional: add Brij 58 to 0.1%. Store dilution buffer at -70 degrees.Proteins are diluted in dilution buffer and quick frozen on dry ice. Thaw proteins on ice. Proteins are typically stable to multiple repeated freeze thaw. 5x binding buffer 1 ml20% glycerol 4
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