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    Gel Mobility Shift Assay

    Contributed by Riddhish Shah, Hueley Lab Gel Preparations:  Assembly for gel casting: Take clean plates and the spacers. Snuggly tighten them to make casting module. Fit it on the stand without the rubber spacer on bottom. Add some water to make sure water is not leaking out at the bottom. Keep the comb handy. Drain off the water by inverting the gel cast. Few droplets sticking to glass plates don’t interfere with gel.  Gel solution preparation and casting:  Water distilled 50 ml30:2 acralamide: bis  8 ml10X TBE  1.5 ml10% APS  600 ul (0.0600 gm in 600 ul)Teme
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