1. RESTRICTION:Use 5mg genomic DNA for Southern hybridization. Aliquot DNA in H20: Total Vol 100l .Add 100l Mix.TOTAL VOL: 200l MIX: Add 100 units per sample of each restriction enzyme.Add appropriate buffer (10X)--20l for each reactionAdd BSA, if needed (100X)----2l for each reactionThen, add H20 for total volume of 100l for each reaction.Example: SacI (20u/l ): 5l NotI(10u/l ): 10l Buffer1(10X): 20l BSA(100X): 2l H20: 63l Incubate overnight at 37° C (or appropriate temp.) in water bath.2. PREC
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