RNA Extraction from Frozen Tissue Sections Tissue Handling: Note that all unfixed human tissue should be handled as BioSafety Level 2 materials (wear gloves, lab coat, etc). All plasticware (tubes, tips, etc.) should be RNase free and always handled with gloves to avoid contamination of the sample with RNases. In general, it is not necessary to treat plastic ware to eliminate RNase contamination- just keep separate, unopened stocks of tubes and tips for autoclaving that are always handled while wearing gloves. Similarly, all chemicals used for RNA should be dedicated for that purpose
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