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实验频道正文 用户登录 新用户注册 DNA电泳 作者：佚名 实验频道来源：中华基因网 点击数： 更新时间：2004-6-8 DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA from Acrylamide Gels DNA Marker Denaturing Gradient Gel Electrophoresis Two Dimensional Gel Electrophoresis DNA Visualization and Staining In Gel Application Migration Table Q&A posted in the method forum Preparation of Agarose Gel and Electrophoresis· Top 10 Fun Facts for DNA Electrophoresis (Produced from LTI)Useful tips for preparing agarose gel and electrophoresis · Agarose Gel Electrophoresis (LaboratoryExperiments.com)A basic and very useful protocol for gel preparation and electrophoresis. · Agarose Gel (John Garland)How to prepare stock solutions and agarose solution · Dissolving and Casting Agarose Gels (FMC)Very nice and detailed procedures for preparing gel and some basic knowledge about agarose gel · Agarose Gel Electrophoresis (Molecular Biology Cyberlab)Very basic and illustrated guide · Agarose Gel Electrophoresis (Julie B. Wolf, UMBC)Provides method in gel preparation, recipes of electrophoresis buffer and loading buffer... · Agarose Gel Electrophoresis (Frank Bottone, Jr.) · DNA Gel (Hoshi Lab)Offers detailed information about resolution of DNA with agarose gel, gel preparation and electrophoresis... · Agarose Electrophoresis (Chen)DNA electrophoresis, extraction and concentration determination. · Agarose Gel Electrophoresis (Roe, OU)· DNA Mobility in Gels (Roe, OU)· Trouble Shooting DNA Agarose Gels (LTI)No band, Smear, anomalies band migration? Find trouble shooting hereDNA Electrophoresis in Agarose Gel (FMC)General and useful tip for agarose gel electrophoresis including the followingBuffers for Electrophoresis Suggested Agarose Concentrations Dye Mobility Tables Running Conditions for Agarose GelsDNA Loading and Loading Buffers Extraction of DNA From Agarose GelTips for Increasing DNA Recovery Efficiency from Agarose Gels (FMC) ß-Agarase Recovery of DNA from Agarose Gels (FMC) Electroelution of DNA from Agarose Gels (FMC) Phenol/Chloroform Extraction of DNA from Agarose Gels (FMC) Modified Freeze/Squeeze Extraction of DNA from Agarose Gels (FMC) DNA Extraction from Agarose Gel (LaboratoryExperiments.com)Protocol for extracting DNA from agarose gel using fileter. Ethanol Precipitation of DNA Recovered from Agarose Gels (FMC) Other Recovery Methods from Agarose Gels (FMC) · Elution of DNA fragments from agarose (Roe, OU)· DNA Extraction from Agarose Gel (Crawford Lab)· Beta-Agarase Gel Purification (Crawford Lab)Recovering DNA from Agarose gel using Beta-Agarase Quick Isolation of DNA From Agarose Gel (Hancock Lab) (Accessible only by Internet Explorer)Centrifugation and subsequent phenol extraction method Isolation of DNA fragments from agarose gels. (Freeze squeeze) (Molecular Genetics Rec Lab) Isolation of DNA fragments from agarose gels. (Freeze spin) (Molecular Genetics Rec Lab) Extraction of DNA from Acrylamide Gels· Isolation of DNA from Acrylamide Gels (Schimenti Lab)· Elution of DNA Fragments from Polyacrylamide Gels (Molecular Genetics Rec Lab)Molecular MarkerFAQs for Molecular Weight Standards and Nucleic Acids (LTI)So many FAQs about molecular weight standards, such as what can I do to prevent DNA standard from smearing? How can I quantitate DNA on an agarose gel? How can I label DNA ladder and more... · DNA Fragment Length Standards (Molecular Genetics RecLab)Lists of DNA restriction fragment length of lambda and pBR322 DGGE· Denaturing Gradient Gel Electrophoresis (DGGE) (Donis-Keller lab)Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially the fragments move according to molecular weight, but as they progress into higher denaturing conditions, each (depending on its sequence composition) reaches a point where the DNA begins to melt. The partial melting severely retards the progress of the molecule in the gel, and a mobility shift is observed. It is the mobility shift which can differ for slightly different sequences (depending on the sequence, as little as a single bp change can cause a mobility shift). Alleles are detected by differences in mobility.Two Dimensional Gel ElectrophoresisStandard 2-D Gel of DNA Replication Intermediatess (Fangman/Brewer)The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of Ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape. Fork-Direction 2-D Gels (Fangman/Brewer)For detecting direction of replication fork movement DNA Staining Visualizing DNA in Agarose Gels (FMC)Provides several detection methods: SYBR® Green I staining, ethidium bromide staining, silver staining and acridine orange and methylene blue staining. · Methylene Blue DNA staining (Gerard Lazo)This method primarily eliminates the damage of DNA by uv irradiation. DNA isolated from methylaene blue stained gels should transform frozen competent E. coli cells on the order of 20-50 fold more efficiently than ethdium bromide isolated DNA. · Vacuum Drying Agarose Gels (FMC)Many people ask how to dry agarose gel, here is the answer In Gel Application A usr/localiety of enzymatic reactions can be performed in the presence of agarose. In-gel reactions are an alternative approach to standard DNA recovery techniques and provide a multitude of benefits. The use of in-gel reactions will not only save time, but eliminate potential sample loss during DNA recovery from an agarose gel (FMC) Tips for Increasing the Efficiency of In-Gel Reactions (FMC) Random-Prime Labeling in the Presence of Agarose (FMC) Cloning in the Presence of Agarose (FMC) In-Gel Cloning (Crawford Lab)Digest your vector and insert DNA and purify by gel electrophoresis and do ligation without further recovering DNA from gel. It works great! Restriction Digestion in the Presence of Agarose (FMC) DNA Amplification in the Presence of Agarose (FMC) Core sample PCR (NWFSC)A method to re-PCR unique bands from products of mixed size Cycle Sequencing in the Presence of Agarose (FMC) Migration TablesDNA Migration in Agarose and Polyacrylamide Gels (Fermentas) Dye Migration in Polyacrylamide Non-denaturing Gels (Fermentas) Dye Migration in Polyacrylamide Denaturing Gels (Fermentas) 处理 SSI 文件时出错 处理 SSI 文件时出错 【评论】【进论坛看】【转入博客】 实验频道录入：生物编辑 责任编辑：生物编辑 上一篇实验频道： DNA抽提 下一篇实验频道： Southen杂交 【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】 相关文章 最新热门 推荐文章 免疫组化染色过程中存在的问常用核酸和氨基酸代码动物模型的设计原则和注意事PCR常见问题总汇如何确定RNA质量的经验谈PCR引物设计的11条黄金法则蛋白纯化经验指南TA克隆常见问题分析及其解决免疫组化实验过程中的要点和核酸的抽提---mRNA的分离技巧 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册 文章评论 （只显示最新10条。评论内容只代表网友观点，与本站立场无关！） 生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助 关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司 公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. 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