RNA电泳-实验方法

实验频道正文用户登录新用户注册 RNA电泳作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 · RNA Gel (Crawford Lab)· Gel Electrophoresis of RNA (Beverly Faulkner-Jones)great tips on RNA gel electrophoresis. · Northern Gel and Transfer (gopher://iubio.bio.indiana.edu)Using glyoxalated RNA· RNA Electrophoresis (Molecular Genetics RecLab) · RNA Electrophoresis in Denaturing Formaldehyde Gel (Molecular Genetics Rec Lab) · Formaldehyde-Agarose Gels (Beverly Faulkner-Jones)Agarose gels have a smaller resolving power than polyacrylamide gels but a greater range of separation - from 200 bp to >50 kb using standard gels and electrophoresis equipment. RNAs up to 10 000 kb can be separated in agarose gels using pulsed field gel electrophoresis. · Denaturing polyacrylamide gel electrophoresis of RNA (Beverly Faulkner-Jones)Polyacrylamide gels have enough resolving power to separate fragments differing by only one base pair in size, but their range is ~ 5 to 1000 bp. They are much more difficult to handle than agarose gels.· Recovery of RNA fragments from polyacrylamide gels (Beverly Faulkner-Jones)The best method is the crush and soak method. It can be sued to isolate single-stranded RNA from denaturing gels. The RNA is of high purity but the method is long and inefficient if long transcripts are to be recovered (cf. less than 30% of DNA greater than 3 kb is recovered).Polyacrylamide Gel Electrophoresis (PAGE) of RNA (Frank Bottone)For use withRibonuclease Protection Assay (RPA): 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：原位转录下一篇实验频道： cDNA 【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章丙烯酰胺凝胶电泳病毒DNA和RNA的简易纯化技术哺乳动物细胞中进行RNA干扰:双向电泳:清洗IEF管银染用考马斯亮蓝R－250染色Poly（A）＋RNA的分离纯化分离纯化总RNA脉冲场凝胶电泳聚丙酰胺凝胶电泳 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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