蛋白质电泳-实验方法

实验频道正文用户登录新用户注册蛋白质电泳作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 蛋白质电泳（主要内容如下）One-Dimensional SDS-PAGE Two-Demensional SDS-PAGE Protein Electrophoresis in Agarose Gel Gel Staining Recipes One-Dimensional SDS-PAGE · Protein Gel and Staining (Gottschling Lab)Provides procedures for gel preparation, gel staining... · Preparation of SDS-Polyacrylamide Gels (SDS-PAGE) (William H. Heidcamp) · SDS Gel Electrophoresis (Petra Klaff)Resolving and stacking gel · SDS-Page Gel Electrophoresis (Dr. Chastain) · SDS PAGE Gels (Gimila Lab) · SDS-Polyacrylamide gels (NWFSC)Standard Laemmli protocol · Denaturing Discontinuous Polyacrylamide Gel Electrophoresis (SDS-PAGE) (Goldberg Lab)Very nice and detailed protocol PolyAcrylamide Gel Electrophoresis (PAGE) gelsPreparation of stock solutions of acrylamide and method of preparation of convex, and gradient gels · SDS-PAGE Gels (PMCI Research)Detailed protocol for preparing gel, reagents for SDS-PAGE and mini-gels · Tricine-Polyacrylamide Gels (NWFSC)For high resolution of small proteins · Tricine SDS-PAGE (Goldberg Lab)Detailed protocol · Electroblotting of SDS-PAA gels (Molecular Genetics Network Lab) SDS-polyacrylamide gels (single or double Minigel system) (Molecular Genetics Network Lab) · Electroelution of Proteins From SDS-PAGE Gels (Mike A. Dyer)This is a general protocol that was developed for the ISCO electroeluter but could easily be applied to other systems. Two-Demensional SDS-PAGE · 2-D PAGE Protein Analysis (ExPASy) o Analytical 2-D PAGE protocols o Preparative 2-D PAGE protocols o Post-separation analysis · Isoelectric Focusing as the First Dimension (UCSF Tumor Immunology) · SDS-PAGE as the Second Dimension (UCSF Tumor Immunology)In this method, proteins are highly denatured and associated with the anionic detergent SDS. Proteins run through the stacking gel, then stack at the membrane where the ion fronts compact them. Upon entering the separating gel, the proteins become separated with lower MW proteins running faster than higher MW proteins. · Analysis of Proteins using Small Format 2D Gel Electrophoresis (Dr Phillip Cash)Detailed protocol on 2D protein electrophoresis. Protein Electrophoresis in Agarose Gel · Protein Electrophoresis in Agarose Gels (FMC)In certain circumstances, electrophoresis of proteins in agarose gels has distinct advantages when compared to polyacrylamide gels. This protocol describes procedures for gel casting, sample preparation, and protein staining and recovery. Gel Staining · SDS PAGE Staining Protocol (LTI)General method for silver stain and coomassie blue stain with recipes · Coomasie Blue Staining of Protein Gels (William H. Heidcamp)Coomasie Brilliant Blue R 250 is the most commonly used staining procedure for the detection of proteins. It is the method of choice if SDS is used in the electrophoresis of proteins, and is sensitive for a range of 0.5 to 20 micrograms of protein. · Silver Staining of Protein Gels (William H. Heidcamp) · Staining of Polyacrylamide Gels (UCSF Tumor Immunology)o Coomassie G250 o Fast Stain (Zoion) o Imidazole-Zinc · Silver staining of PAGE gels · Silver Staining of Acrylamide Gels (Waters Lab) · Silver Stain for SDS PAGE (Hahn Lab) · Silver staining of protein gels · Cupric Chloride Staining : for SDS-PAGE gels (Mike A Dyer)This protocol is 2-3 times more sensitive than coomasie blue staining, it is much quicker, and the gels can be stored at 4° for many months without protein degredation. · Ethanol-based SDS-PAGE Coomassie Blue staining (NWFSC) · Direct Densitometry of Protein Gels (William H. Heidcamp) Recipes · SDS-PAGE Recipes (The Cell Biology and Cytoskeleton Group, HMS) 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：蛋白表达下一篇实验频道：蛋白质相互作用【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章蛋白质提取与纯化技术丙烯酰胺凝胶电泳双向电泳:清洗IEF管银染用考马斯亮蓝R－250染色Poly（A）＋RNA的分离纯化分离纯化总RNA脉冲场凝胶电泳聚丙酰胺凝胶电泳琼脂糖凝胶电泳 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
< 1 > < 2 >

设为首页 | 加入收藏 | 广告服务 | 友情链接 | 版权申明