免疫沉淀-实验方法

实验频道正文用户登录新用户注册免疫沉淀作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 · Analysis of Protein by Immunoprecipitatioin (P.J. Hansen Lab)This protocol describes two methods (protein A and G) in great details as well as the principal, application and trouble shooting of immunoprecipitation. · ECM Immunoprecipitation (LTI)Integrin receptors on cell surfaces are labeled with biotin; the receptors are then extracted from the membranes by detergent treatment and saved for immunoprecipitation. Integrin-specific antibodies are bound to Sepharose beads coated with anti-IgG and added to the biotinylated cell extract. Bound receptors are run on an SDS-PAGE, transferred to nitrocellulose, and detected by the addition of streptavidin-enzyme conjugate followed by substrate. · Immunoprecipitation with anti-Phosphotyrosine - Biotin Conjugates (Transduction Laboratories)Provides detailed protocol on immunoprecipitation assay.· Immunoprecipitation with Soluble Antibodies (Transduction Laboratories)Provides detailed protocol on immunoprecipitation assay.· Immunoprecipitation with Antibody-Agarose Conjugates (Transduction Laboratories)Provides detailed protocol on immunoprecipitation assay.· Immunoprecipitation from Eukaryotic Cell Extracts (PMCI)· Immunoprecipitation and Immune Complex kinase assay (Sefton Lab) · Immunoprecipitation of metabolically labeled cells (Daniel T. OConnor) · Immunoprecipitation (Hancock Lab) · Glycolipid Binding assay (Paller’s Lab, Contributed by Pingsunjim)This protocol can be used for the detection of glycolipids binding to immunoprecipited protein. · Antibody precipitation with Protein A or G (Contributed by James Turmo ) · Immunoprecipitation on Protein A- or Protein G-Coated Plates for Western Blot Analysis 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：蛋白质标记下一篇实验频道：蛋白表达【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章蛋白质提取与纯化技术ImmunoprecipitationCold ImmunoprecipitationsPreparation of Modifed RadImmunoprecipitation of metImmunoprecipitation and ImIMMUNOPRECIPITATIONGlycolipid Binding AssayGeneral Principles of ImmuCO-IMMUNOPRECIPITATION ANA · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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