蛋白质提取和纯化-实验方法

实验频道正文用户登录新用户注册蛋白质提取和纯化作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 蛋白质提取和纯化（主要内容如下）Protein Extraction Protein Purification Protein Precipitation Column Preparatioin Q & A Posted in the Method Forum Protein Extraction Whole Cell Lysate Preparation (UCSF Tumor Immunology)For preparing proteins to be separated by IEF and SDS-PAGE, then sequenced by mass spectrometric techniques. Cell Membrane Preparation (John Garland)Sucrose Gradient and Centrifugation method Protein Extraction (Frank Bottone Jr.) Protein Isolation using Trizol (Frank Bottone Jr.) Protein Isolation From Plants (Gimilas Lab) Subcellular Fractionation of Proteins (PMCI) Protein Purification from Inclusion Bodies (Marks Lab) Preparation of active proteins from inclusion bodies (NWFSC) Preparation of E Coli Protein for SDS-PAGE (Hahn Lab)Simplified method for analyzing E coli protein Protein extracts from Aspergillus mycelium Phenol Extraction of Proteins (LTI)Phenol extraction is frequently used to remove proteins from nucleic acid solutions. Protein PurificationProtein Purification (Michael Blaber)Introduction to protein purification method: Initial Steps of fractionation of protein Protein Purification (Michael Blaber)Guide to protein purification method: Ion exchange elutions, dialysis, concentration · Protein Purification (Michael Blaber)Introduction to protein purification methd: gel filtration, affinity and hydrophobic resins; preparation of resin, plumbing · Purification of GST Fused Proteins (Contributed by Chia Jin Ngee)Many people have vented out frustration over insoluble GST-fused proteins. This is a protocol for enzymatically active soluble GST-fused proteins. All GST-fused proteins are rendered soluble with this technique though enzyme activitiy can range from 30-90%. · GST Fusion Protein Preparation (Vesicle Trafficking)Detailed protocol for GST protein purification. · Green Fluorescent Protein Isolation (Gary J. Lindquester) · GST-Fusion Protein Purification (Marks Lab) · His-tag Protein Purification under Denaturing Conditions (MBS) PPDK His-Tag Purification (Dr. Chastain) PPDK De-Salting (Dr. Chastain) PPDK Purification via Nita Resin Column HeLa Cell Nuclei Preparation (John Garland)Prepare nuclear extract from HeLa cell · Immunopurification of Tyrosine Phosphorylated Proteins (Transduction Laboratories) · Protein Construct Expression and Purification Procedures (Gimilas Lab) · Chloroform Precipitation of Proteins (The Cell Biology and Cytoskeleton Group, HMS)For concentration of proteins from a usr/localiety of aqueous solutions · GST Fusion Protein Purification from Yeast (Dohlman Lab) · Purification of GST Fused Proteins (PMCI) · Sucrose Density Gradient Fractionation of Yeast Membranes (Dohlman Lab) This is an excellent protocol for localization of membrane associated proteins in yeast. · Purification of MBP (maLTose-Binding Proteins) Fused Proteins (PMCI) Purification of His-tagged Proteins from Sf9 Cells under Native Conditions (Brent Graveley) Making Conductivity Curves (Waters Lab)For measuring protein conductivity during protein purification Purificaiton of Protein by Affinity Chromatography (Krause Lab) Protein PrecipitationTCA Precipitation of Proteins (Hahn Lab) TCA Precipitation with DOC (Waters Lab) Protein Precipitation (Gimila Lab) Column PreparatioinMaking Affinity Columns (XMMR) DEAE Column Preparation (Suprya Jayadev) DEAE column: FPLC (Suprya Jayadev) 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道： Western 杂交下一篇实验频道：蛋白质标记【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章蛋白质提取与纯化技术2%多聚甲醛固定剂配方二则细胞学技术细胞培养的一般技术血小板的活化、荧光染色与流血小板活化检测方法细胞表面抗原染色的组织样本流式细胞仪PI染色死细胞方法流式细胞术分析血小板流式检测细胞周期 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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