Western 杂交-实验方法

实验频道正文用户登录新用户注册 Western 杂交作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 Western 杂交（主要内容如下）Preparing of Protein Lysates Western Blotting Far Western Blotting Semi Dry Blotting Stripping Membranes Trouble Shooting and Others Preparing Protein LysatesPreparing Protein Lysates for Western Blotting (Antisense Research Group) Yeast Lysates for Westerns (PMCI) Extracts for Yeast Westerns (Herskowitz Lab Preparation of Brain Membrane Fractions for Western Blot (BD PharMingen) Western Blotting SDS/PAGE Protein Transfer (Gimila Lab) General Western Blot Protocol (KPL) This procedure was written as a general protocol for use with standard Tris-Glycine SDS-PAGE gels and nitrocellulose membranes. It should give acceptable results in most situations. Optimization for particular situations may require modifying the conditions described. · Southerns, Westerns, and Northerns: Molecular Cloning Techniques (Brian White, MIT)Basic theory, definition and procedures with illustration· Western Blotting (Howell Lab)Procedures for preparation of cell lysate, gel, electrophoresis, transfer, antibody detection and stripping membranes.· Western Blots (William H. Heidcamp)Western Blot & Immunostaining (R&D)Provides very nice and general protocol including Western Blot Procedure, immunostaining Procedure (standard and rapid), helpful hints and trouble shooting guide · Western Blotting Protocol (The Cell Biology and Cytoskeleton Group, HMS)· Protein Gel and Staining (Gottschling Lab)· Western Blotting (Corbett Lab) · ECM Protocols Western Blot (LTI)General protocol for ECM assay. A mixture of protein is separated electrophoretically by SDS-PAGE, and the individual protein bands are transferred to nitrocellulose paper. Specific antibody is then used to probe for any of the bands it might bind to, and the nonbound antibody is washed away. The bound antibody is then detected by the addition of a second antibody against the immunoglobulin species contained in the first antibody. This second antibody is labeled with an enzyme, and the specific protein band can then be visualized by the addition of an enzyme substrate containing a color developer.Western Blotting Using Chemiluminscence (Peter J. Hansen Lab) Western Blotting Analysis (BD PharMingen) Western Blotting with Horseradish Peroxidase Conjugates (Transduction Laboratories)Detailed protocol of Western Blotting, from protein isolation, quantification, electrophoresis, transfer and more... Western Blotting with Alkaline Phosphatase Conjugates (Transduction Laboratories)Detailed protocol of Western Blotting, from protein isolation, quantification, electrophoresis, transfer and more... Western Blotting with Monoclonal Antibodies (Transduction Laboratories)Detailed protocol of Western Blotting, from protein isolation, quantification, electrophoresis, transfer and more... Western Blotting with Rabbit Polyclonal Antibodies (Transduction Laboratories)Detailed protocol of Western Blotting, from protein isolation, quantification, electrophoresis, transfer and more... Western Blotting with Biotinylated Antibodies (Transduction Laboratories)Detailed protocol of Western Blotting, from protein isolation, quantification, electrophoresis, transfer and more... · Alkaline Phosphatase (AP) Staining of Western Blots. (Molecular Genetics RecLab) MAP Kinase Gel Shift Assay (PMCI) Anti-Phosphotyrosine Western Blotting (PMCI) · Enzyme-Assisted Immunoelectroblotitng (IEB or Western Blotting) (Molecular Biology Techniques Manual)This protocol describes the use of horizontal blotting of simple SDS-PA gels, and subsequent detection of proteins using rabbit antisera and alkaline phosphatase-conjugated goat-anti-rabbit IgG, detected using bromo-chloro-indolyl phosphate (BCIP) and Nitro-blue tetrazolium (NBT) salts. · Protein Dot-/Slot-Blotting (Schleicher & Schuell)Dot-blots of protein simplify quantitative assays by direct application of the sample to a membrane or filter sheet without prior fractionation. The technique is used for carrying out binding assays on multiple samples, and is valuable for studies in which purification or other processing of the molecule may alter biological activity. The latter advantage makes the dot-blot technique ideal for preliminary screening to determine the effect of blotting or assay conditions on the activity of the sample. · Alkaline phosphatase (AP) staining of Western blots (Molecular Genetics Network Lab) · CDP-Star Western Blot Detection (Gimila Lab) Far Western Blotting · Far Western Blotting (Krause Lab) Semi Dry Blotting · Western Blotting Using the SemiPhor Semi-Dry Transfer Unit (Goldberg Lab)Very nice and detailed protocol with illustration · Semi Dry Blotting · Western Transfer Using a Semidry Apparatus (PMCI) Stripping Western Membranes · Stripping and Reprobing a Western Blot (KPL) · Stripping Western Membranes (Paul Brennan)Reuse blot by stripping antibodies · Stripping Western Membranes (Monica Colitti)Simple protocol for reusing blot Troubleshooting and others · Troubleshooting Western And Dot-Blot Assays (Schleicher & Schuell) · Tips and hints for the storage of antibodies (Synaptic Systems) · Too Many Bands on Western Blot (Synaptic Systems)It is not uncommon that, contrary to the theoretical predictions, several bands are detected. Although it is possible that the antibody is not entirely specific for the protein, other factors may be responsible Western Trouble Shooting (R&D)Offers general guide for Western blotting 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：蛋白质检测下一篇实验频道：蛋白质提取和纯化【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章蛋白质提取与纯化技术基因性状的其它检测方法蛋白质检测－Western BlotWestern杂交Troubleshooting Tips for WTroubleshooting Guide: WesWestern Immunoblotting of Too many bands on a WesterTips and hints for the stoWestern Blot · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. 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