蛋白质检测-实验方法

实验频道正文用户登录新用户注册蛋白质检测作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 · Protein detection (Aberdeens Lab)The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.· Protein detection(Silver staining) (ExPASy)The application of the 2-D PAGE technology to separate, analyze and characterize proteins contained in biological samples would not have been possible without the development of complementary detection methods. Perhaps today one of the most popular non-radioactive protein detection is the silver staining which is 100-fold more sensitive than Coomassie Brilliant Blue staining· Indirect Immunofluorescencc (Brent Graveley) · Coomasie Blue Staining of Protein Gel (William H. Heidcamp)Coomasie Brilliant Blue R 250 is the most commonly used staining procedure for the detection of proteins. It is the method of choice if SDS is used in the electrophoresis of proteins, and is sensitive for a range of 0.5 to 20 micrograms of protein.· CDP-Star Western Blot Detection (Gimila Lab) · Silver Staining of Protein Gels (William H. Heidcamp)· Silver Staining of Acrylamide Gels (Waters Lab)· Silver Staining of Proteins in Gel (Contributed by Sharad Purohit)· Detection of Glycoproteins on Blot (Contributed by Sharad Purohit)· Silver Stain for SDS PAGE (Hahn Lab)· Phosphoamino Acid Analysis of non-TCA Precipitable Proteins (Woodgett Lab) · SDS PAGE Staining Protocol (LTI) 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：细胞核提取下一篇实验频道： Western 杂交【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章蛋白质提取与纯化技术2%多聚甲醛固定剂配方二则细胞学技术细胞培养的一般技术血小板的活化、荧光染色与流血小板活化检测方法细胞表面抗原染色的组织样本流式细胞仪PI染色死细胞方法流式细胞术分析血小板流式检测细胞周期 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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