Northern杂交-实验方法

实验频道正文用户登录新用户注册 Northern杂交作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 Northern杂交· Southerns, Westerns, and Northerns: Molecular Cloning Techniques (Brian White, MIT)Basic theory, definition and procedures with illustrationRNA Gel Preparation· RNA Gel (Crawford Lab)How to prepare RNA gel· Glyoxal/DMSO Gel (Carl Clark)Glyoxal/DMSO gels are useful for determining sizes of RNA especially in conjunction with Northern blots.Probe Preparation· Non-radioactive probes (Gottschling Lab)· Radioactive probes (Gottschling Lab)Northern Transfer and Hybridization· Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel (Contributed by Zuyuan Qian) This is the standard and complete Northern blotting protocol including preparation of RNA gel , RNA sample, and probe, hybridization, detection and recipes for solutions · Northern Blot Preparation (Tyner Lab)Detailed protocol for RNA preparation, gel preparation, electrophoresis, gel staining, transfer and cross-linking...· Northern Transfer Protocol (PMCI Research)· Northern Blots (Carl Clark)Detailed protocol for RNA gel, transfer and hybridization · Northern Blotting (Howell Lab)Detailed protocol for procedures from RNA isolation, sample preparation, to blot hybridization · The Neverfail Northern Blot Hybridization (NWFSC) · Northern Blot (Bowtell Lab)RNA gel and transfer· Northern Blots ( Michael Koelle) (Ambros Lab)· Hybridizing Northern Blots with Riboprobes (PMCI Research)· Northern Blotting (Breeden Lab)RNA electrophoresis, transfer, crosslink and hybridization...· Northern Blotting (Crawford Lab)· Northern Blotting (Gottscheling Lab)· RNA Dot Blot (Crawford Lab)· Northern Blotting (Petra Klaff)complete Northern protocol from RNA gel to blot wash and exposure. · Filter Hybridisation with Radioactively Labelled Probe: Denhardt Method (Molecular Genetics Network Lab)Non-isotopic detectionNorthern Hybridization and Detection (KPL)For chemiluminescent detection of biotinylated probes Cross-Linking· UV Cross-Linking 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道： RNA酶保护试验下一篇实验频道： mRNA末端快速扩增【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章 Southern 杂交鉴定Southern 杂交一般操作Northern Blot实验方法病毒DNA和RNA的简易纯化技术哺乳动物细胞中进行RNA干扰:RNA实验方法培养细胞中RNA检测－NortherRNA点杂交DNA点杂交RNA印迹杂交 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
< 1 > < 2 >

设为首页 | 加入收藏 | 广告服务 | 友情链接 | 版权申明