细菌检测-实验方法

实验频道正文用户登录新用户注册细菌检测作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 Gram Staining (+\-) (William H. Heidcamp) Gram-Staining Procedure (MEDIC, U of Texas)Very nice and detailed method description for Gram staining Acid-Fast Stain (Putts Method)To detect the presence of acid-fast mycobacteria in tissue sections Smear Preparation (MEDIC, U of Texas)The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium. Alkaline Phosphatase and Phospholipase C Assay (Hancock Lab) (Accessible only by Internet Explorer) Bacteria Agglutination Assay (Hancock Lab) (Accessible only by Internet Explorer)Bacterial agglutination is performed using either P. aeruginosa cells autoclaved at 120oC for 1 hour, then centrifuged and resuspended in saline or live P. aeruginosa cells grown to mid log phase, then centrifuged and resuspended in saline. Carbohydrate Assay(Hancock Lab) (Accessible only by Internet Explorer)This assay is used to determine the relative amounts of LPS CHO present in a given strain and can be done on one set of samples and then scanned at the usr/localious wavelengths for reasonable data on the 3 types of sugars. Dintrophenylation Assay for Polymixin B (Hancock Lab) (Accessible only by Internet Explorer) Endotoxin Assay(Hancock Lab) (Accessible only by Internet Explorer)This assay measures the shift in the absorbance maximum of the dye due to binding to endotoxin Indirect Immunfluoresence (Hancock Lab) (Accessible only by Internet Explorer) Liposome Swelling Assay (Hancock Lab) (Accessible only by Internet Explorer) Also including method for liposome Isolation and liposome preparation Preparation of E Coli Protein for SDS-PAGE (Hahn Lab)Simplified method for analyzing E coli protein 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：细菌人工染色体下一篇实验频道：噬菌体的生长【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章发光细菌毒性检测的研究和应菌种保存方法厌氧菌的培养方法测定细菌数量的方法细菌的保存方法美国科学家发现一种用处极大2%多聚甲醛固定剂配方二则细胞学技术细胞培养的一般技术血小板的活化、荧光染色与流 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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