碳水化合物分析-实验方法

实验频道正文用户登录新用户注册碳水化合物分析作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a given strain. The assay can be done on one set of samples and then scanned at the usr/localious wavelengths for reasonable data on the 3 types of sugars. § Sequencing of a Phosphorylated N-Linked Oligosaccharide (Glyko Inc.)§ Enzymatic Sequencing of Lewis Oligosaccharide Structures using FACE (Glyko Inc.)§ Enzymatic Removal of N- & O-Linked Oligos from Glycoproteins (Glyko Inc.)§ A Simple Gel Electrophoretic Method for Analyzing the Muropeptide Composition of Bacterial Peptidoglycan (Glyko Inc.)§ Identification of Glycosaminoglycan Disaccharides (Glyko Inc.)§ Quantitation of Heparin in Human Plasma (Glyko Inc.)§ Sequencing N-Linked Oligosaccharides on rgp120 (Glyko Inc.)§ Sequencing of a Plant N-Linked Oligosaccharide (Glyko Inc.) Micro-Scale Isolation and Purification of Gangliosides (Glycoteck)This solvent partition method permits the isolation of gangliosides from small samples and from samples in which the ganglioside concentration is low, especially relative to the concentration of potentially contaminating proteins and other large molecular weight species. The method consists of three steps: (1) preparation of a dry total lipid extract, (2) partition of the total lipid extract in di-isopropyl ether/1-butanol/aqueous NaCl, and (3) Sephadex G-50 gel exclusion chromatography. The final total ganglioside preparation is lyophilized and stored in the dry state. Immunohistochemistry using Anti-Ganglioside Antibodies (Glycoteck)An indirect method using immuno- fluorescence coupled to second antibody is a relatively simple, rapid, and easy immuno-histochemical procedure. It is best suited for the study of membrane antigens in addition to intra- and extracellular antigens, and may be applied to frozen tissue sections, to cells in suspension, and to cells attached to glass slides or coverslips. Deglycosylation of Glycoproteins Using Endoglycosidases (Glycoteck)Parameters of deglycosylation usr/localy with every glycoprotein and must be determined empirically. Human transferrin is an example of a glycoprotein containing two asparagine-linked alfa-fucosyl-biantennary chains, one of which is only released slowly by endoglycosidases even after denaturation in SDS. A typical test reaction on human transferrin is described in This protocol Immunostaining Thin Layer Chromatograms Of Glycolipids (Glycoteck)This protocol allows for the determination of the number, the size (Rf value) and the charge (combined with DEAE chromatography) of the carbohydrate ligand(s). Furthermore, this method aids in determining the method to use for purification of the glycolipid ligand and subsequently to monitor the purification process Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography (Glycoteck)This protocol describes an effective and simple method including in situ coating an IgG or an IgG-chimeric protein to a perfusion chromatographic column of protein A followed by an optional cross-linking procedure. Dynamic Flow Assay in a Parallel Plate Flow Chamber (Glycotek)Flow assays allow visualization of cell adhesion under well-defined wall shear stress. The visualization of the different events of cell adhesion can be quantified by selective image acquisition and subsequent image processing. Flow assays are uniquely suited to the investigation of adhesive events which occur very rapidly in a time scale shorter than that of most static adhesion assays. In addition, events subsequent to the initial events can be studied such as cell stabilization and spreading giving some insight into the kenetics of particular cell-cell or cell-substrate adhesive behavior. Measurement of Cell Adhesion Under Static Conditions (Glycoteck)This protocol introduces the use of a liquid-filled wash chamber that separates unbound cells by gravity thereby eliminating uncontrolled shear forces and passage of adherent cells through a liquid/air interface. This protocol is also useful for assaying molecules that promote or inhibit cell adhesion. Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates (Glycoteck)his procedure allows detection of specific cell adhesion to glycolipids resolved on TLC plates. The steps include: (1) HPTLC of glycoconjugates, (2) coating the chromatogram with polymer, (3) preblocking the chromatogram to reduce non-specific cell binding, (4) mounting the chromatogram in the acrylic chamber, (5) adding labeled cells, (6) incubating, (7) removal of non-adherent cells by centrifugation, and (8) fixation of adherent cells for detection. 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：酵母双杂交系统下一篇实验频道：细菌培养【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章 2%多聚甲醛固定剂配方二则细胞学技术细胞培养的一般技术血小板的活化、荧光染色与流血小板活化检测方法细胞表面抗原染色的组织样本流式细胞仪PI染色死细胞方法流式细胞术分析血小板流式检测细胞周期检测DNA分析细胞周期 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. 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