酵母人工染色体-实验方法

实验频道正文用户登录新用户注册酵母人工染色体作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 · Easy YAC Preparation Method (Andrew Davies,Shaw lab)· Screening YAC libraries (Donis Keller Lab)This is a method for screening YAC libraries for specific DNA sequences. Transformants are patched onto selective medium plates and colonies lifted to nylon filters for further growth. Cells are then lysed in place and the DNA is fixed to the filters. DNA probes are hybridized to the filters to test for the presence of specific sequences. A secondary screen is performed to ensure a positive hybridization. · YAC library screening by PCR on three-dimensional DNA pools (Stanford)Provides procedure for preparation of DNA Pools and PCR Screening· YAC library screening by hybridization (Stanford)Provides procedure for yeast DNA preparation in MT plates, preparation of hybridization filters, hybridization Preparation of YAC End Probes (Stanford)Procedure for arm rescue Gel-Purified, Agarased YACs for Yeast Transformation (Andreas Gnirke)A protocol for purifying yeast artificial chromosomes by gel electrophoresis and retrieving the resultant products. The products are suitable for use in transformation experiments · Full Scale YAC PLUG Preparation (WUGSC) · YAC Plug 25ml QC Preparation (WUGSC) Restriction Digests of High Molecular Weight Yeast DNA (Donis Keller Lab)Restriction digests of YACs for mapping using rare cutting enzymes or more conventional restriction endonuclease digestion Restriction Digests of YACs in Agarose Plugs (Donis Keller Lab)Restriction enzyme digests on intact, high molecular weight YAC DNA, for use in pulsed-field mapping experiments. Sizing of YAC Clones (Donis Keller Lab)To determine the size of a yeast artificial chromosome within the background of the normal yeast chromosomal complement Subcloning of Yeast Artificial Chromosomes into Phage Lambda (Donis Keller Lab)To subclone the large insert fragment of human DNA contained within a YAC into a bacteriophage lambda vector. Identification of End Clones in YAC Subclone Libraries (Donis Keller Lab)To identify the YAC subclones containing both a human insert and a portion of either the left or right arm of the pYAC4 vector. 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：酵母准备下一篇实验频道：酵母遗传学技术【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章细胞培养的微生物污染排除质粒的酵母直接转化酵母遗传学方法12：酵母菌落酵母遗传学方法11：PCR介导基酵母遗传学方法10：固定化细酵母遗传学方法9：酵母免疫荧酵母遗传学方法8：酵母活体染酵母遗传学方法7：随机孢子分酵母遗传学方法6：羧肽酶Y的酵母遗传学方法5：酵母β－半 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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