酵母准备-实验方法

实验频道正文用户登录新用户注册酵母准备作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 Yeast DNA Preparation Yeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (rapid glass bead prep) (Hahn Lab) Yeast Genomic DNA Preparation (high quality DNA) (Hahn Lab) Yeast quick plasmid DNA Prep (Hahn Lab) Yeast smash-&-grab DNA Preparation (Fangman/Brewer lab) Yeast Genomic DNA Preparation for 2-D Gels (Fangman/Brewer lab) Preparation of Yeast DNA Embedded in Agarose Plugs (Fangman/Brewer lab) Yeast Genomic DNA Miniprep (Breeden Lab) Yeast Chromosome DNA Preparation (Breeden Lab) Yeast DNA Minipreps (Donis Keller Lab)Quick method for preparing yeast genomic DNA High Molecular Weight Yeast Liquid DNA Preparation (Donis Keller Lab)For isolating intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 µg of DNA per prep. Crude Purification of PCR-able DNA Directly from Yeast Cells (GSCWU) Restriction Digests of High Molecular Weight Yeast DNA (Donis Keller Lab)Restriction digests of YACs for mapping using rare cutting enzymes or more conventional restriction endonuclease digestion. Isolation of Yeast Genomic DNA (D. Amberg) DNA Isolation from Yeast (Corbett Lab) Yeast Miniprep (Corbett Lab) Yeast RNA PreparationYeast RNA Preparation (Gottschling Lab)Give protocol for RNA preparation using phenol/freezer method and glass bead method Yeast RNA Miniprep (Breeden Lab) Yeast Protein PreparationYeast Protein Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Whole Cell Extracts (Dohlman Lab)This is a quick method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting. Quick Total Yeast Protein Isolation for SDS-PAGE Gels (Dohlman Lab)A quick method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting (same protocol as above) Yeast Protein Preparation for SDS PAGE and Western (rapid) (Haha Lab) Yeast Protein Preparation for SDS PAGE and Western (Haha Lab)Yeast whole cell protein extraction for western blot using glass bead method · Sucrose Density Gradient Fractionation of Yeast Membranes (Dohlman Lab) This is an excellent protocol for localization of membrane associated proteins in yeast. · Protein Extraction from S. cerevisiae (Howell Lab)Glass bead method · Extracts for Yeast Westerns (Herskowitz Lab) · Yeast Protein Isolation (Amberg Lab)Yaffe-Schatz method · Yeast Nuclear Extract Prep (small scale) (Hahn Lab) · Yeast Whole Cell Extract (small scale) (Hahn Lab) 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：酵母培养基和相关试剂下一篇实验频道：酵母人工染色体【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章细胞培养的微生物污染排除质粒的酵母直接转化酵母遗传学方法12：酵母菌落酵母遗传学方法11：PCR介导基酵母遗传学方法10：固定化细酵母遗传学方法9：酵母免疫荧酵母遗传学方法8：酵母活体染酵母遗传学方法7：随机孢子分酵母遗传学方法6：羧肽酶Y的酵母遗传学方法5：酵母β－半 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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