足迹法(Footprinting)-实验方法

实验频道正文用户登录新用户注册足迹法(Footprinting) 作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 Footprinting Procedures· DNase I Footprinting (Mike A. Dyer)· DNase I footprintingDetermining the site of binding for a protein on a DNA sequence· DNase I Footprinting (Bowtell Lab)· DNase I Footprinting (Crawford Lab)Detailed protocol for footprinting, including strategy for probe labeling, recipes and more...· Exonuclease III footprinting· Hydroxyl radical footprinting (TTO)(To view this article, registration is needed, but free)Hydroxyl radical footprinting is a powerful technique for investigating DNAprotein interactions. In contrast to DNase I footprinting, it allows the exact determination of contact sites in the DNA target sequence. This is a modification of this method in which the DNA fragments for the footprint assay are non-radioactively labeled and generated by PCR. The fragments are analyzed after incubation with the specific DNA-binding protein and subsequent treatment with hydroxyl radicals, on an ALFexpress DNA SequencerOther procedures· DMS-treating DNA (Indiana U)DMS treatment under controlled conditions, followed by piperidine cleavage, yeilds a ladder of fragments indicating the positions of all G residues in the DNA sequence. These ladders are useful MW standards for S1 experiments, footprinting, etc.· Preparation of G+A Marker (Technical Tips Online)A rapid one-step procedure for preparation of G plus A sequence markers by acid-induced apurinization and DNA cleavagePreparation of G+A MarkerSimplified method for preparing G+A ladder run along with footprinting reaction. Its much simple than the original Maxima-Gilbert sequencing reaction and works fine. 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：条带转移(Band Shift) 下一篇实验频道：蛋白质制备【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章免疫组化染色过程中存在的问常用核酸和氨基酸代码动物模型的设计原则和注意事PCR常见问题总汇如何确定RNA质量的经验谈PCR引物设计的11条黄金法则蛋白纯化经验指南TA克隆常见问题分析及其解决免疫组化实验过程中的要点和核酸的抽提---mRNA的分离技巧 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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