M13噬菌体-实验方法

实验频道正文用户登录新用户注册 M13噬菌体作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 · M13 Phage (Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phage culture, it will be great beneficial to read this.· Large Scale M13RF Isolation (Roe Lab)After the M13RF containing bacterial cells are harvested by centrifugation, the double-stranded molecules are isolated using the cesium chloride method as for large scale plasmid isolation...· Phage Miniprep An improved method for small scale prep. of bacteriophage DNA based on phage precipitation by zinc chloride.· Single-Stranded M13 DNA Isolation Using Phenol (Roe Lab)This isolation procedure is the method of choice for preparation of M13-based templates to be used in dye-terminator sequencing reactions. · Preparation of Single Stranded M13 Template DNA (ScienceXchange)· PCR of M13 inserts: (GSC, WU) · Preparation of M13 sublibraries: (GSC, WU) · Double Stranded DNA Isolation using Phenol(Whitehead Institute/MIT)· Phagemid Purifications using Streptavidin-coated Magentic Particles(Whitehead Institute/MIT)· M13 Purifications using Streptavidin Coated Magnetic Particles (Whitehead Institute/MIT)· M13 RF prep large-scale: (GSC, WU) · M13 ThermoMAX prep: (GSC, WU) · M13 helperphage: (GSC, WU) · M13 PEG prepI: (GSC, WU) · M13 PEG prepII: (GSC, WU) · Large-scale M13 prep: (GSC, WU) · Single Stranded Template from pBluescript Phagemid (NMFS/NOAA) · Phage Culture and DNA Isolation (ScienceXchange)· Figure-8 Test (Jim Brown)The figure-8 test is a test that identifies M13 clones that contain complementary sequences in their viral ssDNA. This occurs when the two clones contain the same region of DNA inserted in opposite orientations. This can be used to identify clones containing the same DNA fragment inserted in opposite directions, to identify clones containing nonidentical but overlapping complementary regions, or to find clones containing portions of DNA also found (complementary) in a larger clone. · Single Stranded DNA Preparation (Crawford Lab)· Preparation of ss Phagemid DNA(mini and maxi prep) (Life Technologies)The protocols can be used to prepare ssDNA from DH5aF, DH5aFIQ, DH11S, or DH12S cells.· Phage Purification with DE52 (PMCI Research) · Colony/Plaque Blotting (Gimilas Lab) 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道： Lambda噬菌体下一篇实验频道：条带转移(Band Shift) 【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章 Single-stranded DNA preparM13 PhageHigh-throughput preparatioLarge scale M13RF isolatioISOLATION OF SS DNA FROM BSingle-stranded M13 DNA isPhage DNAPhage TiterBacteriophage PreparationPreparation of phage parti · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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