噬菌体的生长-实验方法

实验频道正文用户登录新用户注册噬菌体的生长作者：佚名实验频道来源：中华基因网点击数：更新时间：2004-6-8 Preparing Lawn Cells for M13 Cloning (Life Technologies)Lawn cells require the F episome for M13 infection and may be prepared Streaking Lambda Phages (Donis Keller Lab)Phage are streaked onto a medium to obtain an independent isolate prior to preparing a new lysate Phage Propagation (hancock Lab) (Accessible only by Internet Explorer) Helper Phage Preparation (Vesicle Trafficking) Picking Single Phage Plaques(Donis Keller Lab)For isolating single bacteriophage lambda plaques to be used for making lysates Lambda plaque screening (Molecular Genetics Network Lab) Liquid Phage Lysates (Donis Keller Lab) Preparation of Phage Lysates (Goldberg Lab)Very nice and detailed protocol including recipes for media preparation Gel Assay to Determine DNA Content of Phage Lysates (Donis Keller Lab)A crude lysate gel assay can be performed to roughly quantitate the DNA in lysates. This is often a valuable time saving step to determine if the phage yield is sufficient to warrant continuing the procedure Titering Lambda Phages (Donis Keller Lab)This procedure is used to find the number of viable phage particles in a lysate. The phage lysate is serially diluted and ... Phage Plate Stock Lysates (Donis Keller Lab)To prepare phage lysates to be used for small or large scale phage DNA preps. This method usually produces lysates with titers of 2-8x10e10 pfu/ml. Long-Term Lambda Phage Storage (Donis Keller Lab)Store phage in 7% DMS0. Phage and Cell Storage (Gimila Lab) Transfer of Phage DNA from Plaques to Nylon Membrane or Nitrocellulose (Donis Keller Lab)To transfer phage DNA to nylon membranes so that a phage library can be screened by hybridization Assay for Phage Containing the ß- Galactosidase Gene (Donis Keller Lab)This assay is used when working with phage vectors carrying the beta-galactosidase gene (often used for immunological screening). If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear in the assay. If the phages contain a functional beta-galactosidase gene they will form blue rings around their plaques. Plaque Lifts (Gimilas Lab) Identifying Plaques by Neutral Red Staining (LTI) Phage Titre and Solution Preparation (Gimilas Lab) Colony/Plaque Blotting (Gimilas Lab) Calculating Phage Titers (Goldberg Lab) Phage Media Diagram (Goldberg Lab)procedures for phage media preparation Growth and Purification of 25-100 µg Lambda Clone DNA (Donis Keller Lab) Lambda Methods Media and Solutions (Donis Keller Lab) Isolation of Phage Resistant Mutants (Hancock Lab) (Accessible only by Internet Explorer) 处理 SSI 文件时出错处理 SSI 文件时出错【评论】【进论坛看】【转入博客】实验频道录入：生物编辑责任编辑：生物编辑上一篇实验频道：细菌检测下一篇实验频道：噬粒（phagemid）【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】相关文章最新热门推荐文章 Phage DNAPhage TiterBacteriophage PreparationPreparation of phage parti2%多聚甲醛固定剂配方二则细胞学技术细胞培养的一般技术血小板的活化、荧光染色与流血小板活化检测方法细胞表面抗原染色的组织样本 · 免疫细胞表面抗原分子CD家族对照表· 所有的看家基因(housekeeping gene· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· 重组DNA的分离、克隆与测序实验手册· RNAi和基因沉默的历史回顾· RNAi原理图解· RNAi术语表· siRNA设计指南· Methods for DNA sequencing · 通用电气医疗集团简介· 生物发光与化学发光专题· 生物发光和化学发光在生物技术中的· RNA干扰技术获得新突破· 蛋白质特性与分离纯化技术的选择· PCR实验指导与常见问题分析· RNAi原理FLASH演示· Extrachromosomal elements, plasm· Promoter analysis by saturation · 重组DNA的分离、克隆与测序实验手册文章评论（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）生物谷 | 网站介绍 | 网站地图 | 广告服务 | 帮助关于我们 | 广告服务 | 战略伙伴 | 留言| 帮助信息| 联系方式 | 客户投诉 | 友情链接 | 网站地图 | 网站声明 | Bioon English 上海北岸信息技术有限公司公司地址：上海龙吴路51号嘉源商务中心1#211室(200232) 生物谷网站 bioon.com © Copyright 2001 - 2006. All rights reserved. 备案号：沪ICP备05022939号
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