常规杂交反应存在的问题

交的残留核酸样品。但这种常规杂交方式在速度、灵敏度和特异性等方面都阻碍了整个芯片的杂交效果。因为，探针必需具有相似的解链温度，这样才能保证杂交条件的一致性。杂交反应与溶液中靶序列的初始浓度成正比，所以必需提高靶DNA的浓度以加快反应速度。 [参考文献] 1. Bains W. Selection of oligonucleotide probes and experimental conditions for mμltiplex hybridization experiments. Genet Anal Tech Appl, 1994;11(3):49-62. 2. Honore B, Madsen P. The tetramethylammonium chloride (TMAC) method for screening cDNA libraries with highly degenerate oligonucleotide probes obtained by reverse translation of amino acid sequences. Methods Mol Biol, 1997;69:139-46. 3. Honore B, Madsen P, Leffers H. The tetramethylammonium chloride method for screening of cDNA libraries using highly degenerate oligonucleotides obtained by backtranslation of amino-acid sequences. J Biochem Biophys Methods,1993; 27(1):39-48. 4. Oh SH, Fleischhauer K, Yang SY. Isoelectric focusing subtypes of HLA-A can be defined by oligonucleotide typing. Tissue Antigens 1993 Mar;41(3):135-42.
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