Serum Protein Electrophoresis Tricine/Polyacrylamide Gel ElectrophoresisUsed for pilin processing analysis but generally useful for resolution of small (15-35 Kdal) proteins of similar size. SeeStrom, M. S., D. N. Nunn, and S. Lory. 1993. A single bifunctional enzyme, PilD, catalyzes cleavage and N-methylation of proteins belonging to the type IV pilin family. Proc. Natl. Acad. Sci., 90:2404-2408. or Strom, M. S., and S. Lory. 1992. Kinetics and sequence specificity of processing of prepilin by PilD, the Type IV leader peptidase of Pseudomonas aeruginosa. J. Bacteriol. 174:7345-7351.
< 1 > < 2 >
|
