Purification of plasmid DNA (miniprep) with high yields using diatomaceous earthKyung-Soo Kim and Charles K. PallaghySchool of Botany, La Trobe University, Bundoora Vic 3083, AustraliaCorrespondence to C. K. Pallaghy, e-mail [email protected] Copyright K-S. Kim and C. K. Pallaghy; 1996, All rights reserved. Modified 3/10/97IntroductionThis cheap and simplified protocol, based on Hansen et al., 1995, gives high yields of plasmid DNA as well as high purity and is suitable for cloning, PCR, sequencing, site-directed mutagenesis and in vitro transcription, etc. It is an extremely good
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